Date of Award
12-2016
Degree Type
Thesis
Degree Name
Master of Science
Department
Clinical Sciences
Program
Clinical Molecular Genetics [discontinued]
First Advisor/Chairperson
Dr. Paul Mann
Abstract
Staphylococcus aureus and Streptococcus pneumoniae are bacteria that commonly colonize healthy individuals without causing disease. Methicillin-resistant S. aureus (MRSA), a more virulent type of S. aureus, is carried by a small percentage of people. These two bacteria have an adversarial relationship both in vitro and in vivo, with S. pneumoniae being able to limit the growth of S. aureus. It has been hypothesized that the relationship between these bacteria may be altered in individuals immunized with the pneumococcal conjugate vaccine, raising concerns that vaccinated individuals may be more likely to carry MRSA. Assessing the carriage rate of these bacteria in both vaccinated and non-vaccinated individuals may provide important information to support or refute this hypothesis. To facilitate this study, we developed a multiplex Real Time Polymerase Chain Reaction (PCR) assay to simultaneously detect S. aureus and S. pneumoniae in the same sample.
Recommended Citation
Pierpont, Melissa J., "MULTIPLEX REAL TIME PCR and MELT CURVE ASSAY DEVELOPMENT FOR THE SIMULTANEOUS DETECTION AND IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE and STAPHYLOCOCCUS AUREUS" (2016). All NMU Master's Theses. 116.
https://commons.nmu.edu/theses/116
Access Type
Open Access