Date of Award


Degree Type


Degree Name

Master of Science





First Advisor/Chairperson

Katherine Teeter

Second Advisor

Kurt Galbreath

Third Advisor

Alec Lindsay


American pika (Ochotona princeps) are small winter-active mammals inhabiting alpine environments. As the American pika occupies a niche characterized by hypoxia, these animals are an excellent system to use for detecting genetic changes associated with adaptation to these conditions. American pika populations were located at lower elevations during glacials, and shifted upslope during interglacials. In this scenario, pikas would have experienced increased selective pressure to develop adaptations to hypoxia. Here, I use a candidate gene approach to identify molecular signals of adaptation in the American pika genome. I first examined the sequences of 54 candidate genes for adaptation in two subspecies of O. princeps using draft genomes available from public databases. I constructed phylogenies using the two O. princeps alleles and homologs from other mammal species, and then used the CODEML module of PAML to detect evidence of selection along branches in the phylogeny. Four genes (EHHADH, IGF-1, TGF-β1, and NOS2) showed evidence of adaptation in these analyses. I then resequenced portions of these four genes in 25 individuals from across the species’ range, and analyzed the resulting data using frequency-based tests of non-neutral evolution. These tests have provided additional support for positive selection in the EHHADH, IGF-1, and TGF-β1 loci. These loci represent strong candidates for further study of adaptation in this system, including examination of their functional role in the response to hypoxia.