The current worldwide pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) produces an urgency to find a way to quickly test people for the virus. Currently a Real Time Polymerase Chain Reaction (RT-PCR) assay is the “gold standard” test to identify infected individuals. Therefore, I am seeking to use Loop Mediated Isothermal Amplification (LAMP) as an assay to detect SARS-CoV-2. LAMP offers greater specificity and sensitivity compared to PCR. The overall objective will compare and characterize Dr. Jennings’ designed LAMP primers with published primer sets with confirmation of successful LAMP amplification via RT-PCR and gel electrophoresis. Using the products of LAMP, a RT-PCR shall confirm that SARS-CoV-2 amplification occurs reproducibly. Confirmation relies on a restriction enzyme digest to see whether the RT-PCR and LAMP reactions produced SARS-CoV-2 RNA. This project will help confirm that the Primers Dr. Jennings designed will detect SARS-CoV-2 RNA.
Dr. Matthew Jennings
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Rose, Scott Z., "Characterization of LAMP Primer Design Compared to Published Results" (2021). Celebration of Student Scholarship. 30.