Date of Award

4-2024

Degree Type

Thesis

Degree Name

Master of Science

Department

Biology

Program

Biology (MS)

First Advisor/Chairperson

Dr. Alan Rebertus

Abstract

Bdelloid rotifers are well documented as inhabitants within lobules of the widely distributed epiphytic liverwort, Frullania eboracensis. This research provides a detailed description of rotifer occupancy within lobules of F. eboracensis and tests the hypothesis that rotifer excrement might be a source of nutrients for F. eboracensis. My goals were to 1) investigate how rotifers are distributed among F. eboracensis, 2) how rotifer abundance is impacted by seasonality, and 3) use quantum dot (QD) labeled Escherichia coli as a food source for rotifers and track the QDs from bacteria to rotifer to F. eboracensis. Frullania eboracensis samples were collected in fall 2014 to fall 2016. QD experiments were conducted in fall 2023. The results showed rotifers had a much higher probability of occupancy for the first 5-6 lobules, slowly declining thereafter. Rotifer abundance was stable across the 1.5-year period, but there was a slight increase in density during the late fall and early winter. QD fluorescence was observed within the stomach cavity and intestine of rotifers and F. eboracensis showed evidence of rotifer-derived QD uptake from rotifer feces. The findings in this study support the hypothesis that F. eboracensis utilizes nutrients from rotifer feces and is the first study to document transfer of QDs from bacteria to animal to liverwort.

Access Type

Open Access

Justification for Restricting Access

I am requesting an embargo for my thesis because I am working on submitting the manuscripts for publication.

Available for download on Monday, May 07, 2029

Share

COinS