Date of Award

5-2025

Degree Type

Thesis

Degree Name

Master of Science

Department

Biology

Program

Biology (MS)

First Advisor/Chairperson

Dr. Donna Maki

Abstract

Regional food insecurity caused by the inability to use conventional farming techniques year-round in cold climates increases the need for access to quality, locally sourced, and affordable foods year-round. Shifting from a soil-based system to aeroponics can increase yields, as aeroponic systems are not weather-reliant and can operate year-round. Aeroponics, a subgroup of hydroponics, induces growth by misting nutrient-rich solutions onto the plant roots. These systems use space and water more efficiently and rely less on fertilizers and pesticides but are vulnerable to errors in nutrient application, power loss, and rapid pathogen spread. Adding Vermicompost (VCT) and plant growth-promoting microorganisms (PGPM) offers a potential solution by improving nutrient efficiency, resulting in higher yields, improved nutritional value, and reduced pathogenesis. Spinach was selected as the study organism because it is consumed globally due to its rich nutrient value and palatability. Adding VCT and PGPM resulted in 31.3% higher germination rates and reduced pathogen prevalence. The addition of PGPM and VCT did not impact tissue yield. The mineral content of leaf tissue was highly variable, with soil treatments having the most positive and negative significant mineral values. Aeroponic systems produced mineral values well above the recommended, with less variability. Exploring the interaction of aeroponic systems with PGPM and organic amendments will help develop sustainable agricultural methods for communities with reduced agricultural prospects due to land availability or climate.

Access Type

Open Access

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