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This study investigated the within-day repeatability and between-subject variation of vector coding variability during treadmill running. The lower-limb kinematics of eight male triathletes running at 12 km/h were captured twice on the same day. Mean coupling angle variability across the stride cycle was computed for 20 couplings using the modified vector coding technique. Between-subject variation was calculated from the first data collection and data from both sessions were used to calculate systematic and typical error. Between-subject variation ranged from 1.4° to 3.7°. Systematic errors between sessions were observed for two couplings and typical errors were between 0.6° and 1.9°. The between-subject variation and within-day repeatability of the measures reported will aid the interpretation of vector coding variability in cross-sectional and intervention studies.