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Active workstations make it possible to interrupt prolonged sitting during the office worker’s day, alleviating some health risks. Interrupting prolonged sitting with use of a treadmill desk revealed positive effects on postural stability. The present study aimed to investigate the acute effect of breaking up prolonged sitting with body weight resistance exercise on postural stability. Eight sedentary adults participated in a randomised two treatment crossover design: 1) uninterrupted sitting for 5 hrs versus 2) interrupting sitting with 3 min of exercise every 30 min. On a pressure plate, postural stability tests were performed pre and post condition. Anteroposterior centre of pressure (CoP) amplitude and CoP distance showed a significant interaction in the bipedal eyes open test; use of resistance exercise decreased postural stability. This suggests other active workstations (e.g. walking desks) may be more effective.