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The purpose of this study was to clarify the difference between normal sprinting (NS) and running over flat markers (FMR). Seven male collegiate sprinters participated in this study as subjects. The subjects initially ran 50m normally, then over some markers set up on the runway, and finally they ran 50m normally again. The leg motion of the three runs was compared by two-dimensional motion analysis. FMR showed a significant increase in stride frequency and running velocity. Leg motion had no significant difference. However, knee torque and torque power showed a significant difference. The results suggested that the second sprint was influenced by FMR. FMR may be a good tool for sprinting improvement to obtain a higher stride frequency.