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The purpose of the this study was to the change of direction (COD) movement in the backward direction during the defensive phase. Thirteen male soccer players performed the reactive agility test (RAT) and sprint running. Analysis classified the players to two groups according to the RAT time. There was no significant difference in sprint times, but RAT of the fast group was significantly faster than that of the slow group. Before and after COD of RAT, the fast group had made a deceleration quickly by high-step frequency. In the COD movement, the fast group performed the movement with their body tilted backward while lowering their center of gravity with the hip of the COD foot bent before COD and smaller knee flexion displacement during COD support phase. These findings seem to be a basic knowledge for evaluation and training of COD in soccer.