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The aim of this study was to compare isometric strength asymmetry in participants that have previously undergone anterior cruciate ligament (ACL) reconstruction and a healthy control group. Three-dimensional force data (1000 Hz) were collected from 21 ACL (3.2 ± 1.8 years post-surgery) and 21 control participants during maximal isometric contractions. Peak knee flexion force displayed significant asymmetry differences between groups, with ACL participants showing greater asymmetry (7.6 %) than the control group (0.1 %). No significant asymmetry differences were found between groups for peak extension, adduction and internal rotation force. Results suggest that following ACL reconstruction and rehabilitation, external force production during knee flexion is significantly less on the affected side than the uninjured side, which has implications on rehabilitation monitoring.