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The purpose of this study was to examine the running kinematics such as stride length, stride rate, speed, and joint range of motion when trunk rotation was manipulated. Six female recreational runners were recruited to perform a total of nine trials of over ground running in their comfortable speed (≈ 3.31 m/s) for natural gait, restricted trunk rotation, and exaggerated trunk rotation conditions. Exaggerating trunk rotation during running resulted in greater stride length and smaller stride rate significantly when compared to natural and restricted trunk rotation conditions. Although some of the significant change of the running kinematics were observed, the manipulation of the trunk rotation did not alter the overall running kinematics such as speed and range of motion at hip, knee, and ankle joints during braking and propulsive phases.