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The purpose was to compare pre-landing and early landing knee biomechanics between males and females and between double-leg and single-leg landings. Sixteen males and sixteen females participated in this study. The single-leg landings generally resulted in less time to minimal knee flexion angles prior to initial landing, decreased minimal knee flexion angles and average knee flexion velocities prior to initial landing, knee flexion angles at initial landing. The sex difference was only observed for knee flexion angles 50ms after initial landing in single-leg landings. These differences in the timing of minimal flexion angle and minimal knee angle prior to landing might be related to a small knee flexion angle at initial contact. Preparing for landing with greater knee flexion help mitigate the ACL injury risk in single-leg landings and women.