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This study compared normalization methods for surface electromyography (sEMG) for comparing individuals with (Para) and without (AB) paraplegia. Participants (Para, n=7, AB, n=11) performed 4 minutes of arm-cycling at several submaximal intensities, and an incremental maximal test to exhaustion, while sEMG of the right biceps brachii was recorded. This study analyzed sEMG at two intensities: rate of perceived exertion (RPE) 13 and at 60 W, with four methods of normalization: non-normalized, against a maximal voluntary contraction (MVIC), against a rate of perceived exertion (RPE) 9, and against the max test. Using submaximal exercise intensity based on RPE or power output will affect the results when comparing sEMG of Para and AB groups, regardless of which normalization method is used to inspect the data.