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The purpose of the present study was to verify the reliability of the prediction equation for interval time (the times between hurdles) established from the hierarchical model in the 400 m hurdles. From 155 data samples of previous studies (record range: 47.42–50.99s), regression equations were established to predict each interval time throughout the race (model #1), during the first and latter halves (model #2) and during the first, middle, and last parts (model #3). Leave-one-out cross-validation was used to avoid overtraining for each regression equation. Models #2 and #3 showed better goodness of fit and generalization for each interval time compared to model #1. These hierarchical models are found to be more reliable compared to the non-hierarchical model. The hierarchical model will help to set the pace distribution to achieve the athlete’s target records accurately.