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Stride phase characteristics have been identified as contributors to pitch velocity. The aim of this study was to determine how in-game pitching stride phase characteristics and pitch velocity changed across a pitching appearance. Five (5) male Division-I pitchers were measured across an appearance during a collegiate baseball game. Five (5) biomechanical characteristics during the stride phase and pitch velocity were assessed. Biomechanical and pitch metrics were collected for each pitch and averaged per inning. Center of Mass Anterior/Posterior Velocity at Foot Contact was observed to significantly increase across time, though pitch velocity did not change across time. This may suggest that pitch velocity could be a trailing indicator of mechanical change. If true, biomechanical data should be leveraged to better identify when pitching biomechanical patterns may be changing during an outing.